Fig. 1

Localization experiments of chs7305. a Confocal fluorescence microscopy images of T. pseudonana transformants expressing the gene of the C-terminal chitin synthase 7305 fusion protein chs7305-CT-GFP and the corresponding Western blot results against GFP. The micrograph is an overlay of the recorded green channel (excitation: 488 nm, emission: 490–536 nm range) and the red channel (excitation: 633 nm, emission: 647–687 nm range). The green color represents GFP fluorescence, whereas the red color is caused by chloroplast autofluorescence. b Western blot analysis (against GFP) of T. pseudonana transformants expressing the gene of the chitin synthase 7305 fusion protein chs7305-ED-GFP (159 kDa) with the GFP gene within the extracellular domain E1. c Schematic representation of the domain structure of chs7305 (132 kDa) and the locations of recombinant GFP introduction (green arrows). C: cytosolic domain, E: extracellular domain, TM: transmembrane domain